Taq polymerase is used to amplify the cDNA through denaturation, annealing and extension steps.

Taq聚酶通过变性、退火、延伸等步骤用于扩增cDNA。

Once we've got the cDNA, the steps of RT-PCR are pretty much the same as PCR.

一旦我们获得了互补DNA，RT-PCR步骤便和PCR相似。

If the viral RNA is present, the primers attach to the RNA strand and then reverse transcriptase synthesizes a cDNA strand.

如果存在相关病毒RNA，引物便会与RNA单链结，逆转录酶将会成一条互补DNA链。

Julius, he started with a cDNA library, it's called, it's copies out of all proteins that are synthesized in a cell.

朱从 cDNA 文库开始，它是从细胞中成所有蛋白质中复制出来。

Initially, production in bacteria was the only route available and cDNA clones were used as they contained no introns.

cDNA需要与原核生物转录和翻译信号相连，并且需要插入多拷贝质粒。

RNA is complimentary to DNA though, so if an RNA strand has a nucleotide sequence 3' - A A G U C C A G U - 5', then the complimentary DNA or cDNA would be 5' - T T C A G G T C A - 3'.

但RNA与DNA间互补，所以如果一条RNA链拥有如下核苷酸序列：3' - A A G U C C A G U - 5'，那么与互补DNA便是5' - T T C A G G T C A - 3'。