Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷消化法
离出尿路上皮细
，
于无血清生长
。
Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷消化法离出尿路上皮细，于无血清生长。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚
离成纤维细,
采用细悬液法和胰酶消化植块法对其进行。
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